S430 User Manual
57
K
1
∑
A
i
C
i
1
n
∑
A
i
∙
∑
C
i
n
i1
n
i1
n
i1
∑
A
i
2
1
n
∑
A
i
n
i1
2
n
i1
K
0
∑
C
i
n
i1
n
K
1
∑
F
i
n
i1
n
6.2.2 Quadratic Working Curve (2nd order)
The calculation formula is as follow:
2
2
1
0
Where,
C: Concentration of standard sample
A : Abs. of each sample (measured value)
Kn are calculated by the least squares method
Suppose there are n data points(
A
n
,C
n
),then:
K
2
SA
2
CS
AA
S
AC
SAA
2
S
AA
SA
2
A
2
SAA
2
2
(Formula F5-5)
K
1
S
AC
SA
2
A
2
SA
2
CSAA
2
S
AA
SA
2
A
2
SAA
2
2
(Formula F5-5)
K
0
∑
C
i
n
i1
n
K
1
∑
C
i
n
i1
n
K
2
∑
F
i
2
n
i1
n
(Formula F5-7)
6.2.3 The correlation coefficient
The correlation coefficient R represents how the regression curve fitting.
Suppose there are n data points
,
:
∑
1
∑
1
∙
∑
1
∑
2
1
∑
1
2
∑
2
1
∑
1
2
(Formula F5-13)