S430 User Manual
6
1 Instrument Overview
1.1 Theory
The principle of spectrophotometric analysis is to show the substances absorb
phenomenon on different wavelengths and do qualitative and quantitative analysis of the
substances.
This instrument is measuring base on relative measurement. Choose a certain
substance (Distilled water, air or the sample) as reference solution, set its transmittance
to 100%. The transmittance of the sample being tested is relative to the reference
solution. The transmittance and concentration of the test substance is related. Within a
certain range, it is consistent with Lambert - Beer law.
T=I/Io
A=KCL=‐㏒ I/Io
T :Transmittance
A: Absorbance
C: Concentration
K: Absorption coefficient of the solution
L: Length of the liquid layer in the optical path
I: Intensity of light transmitted through the test sample on the photoelectric
converter
Io: Intensity of light transmitted through the reference sample on the photoelectric
converter
This instrument is widely used in medicine and health, clinical testing,
biochemical, petrochemical, environmental monitoring, food production and
quality control departments for qualitative and quantitative analysis. It is also
as a teaching demonstration and laboratory equipment, and post-secondary
institutions and related courses.
The S430 Near-Infrared Spectrometer is a grating-type near-infrared
spectrometer. Wavelength range is 1000nm-1800nm. This instrument is
mainly used for non-destructive testing of liquid samples. The analysis
process is very convenient, Just put the cuvette filled with samplesinto the
instrument sample compartment, click on the PC software to get the sample
spectral data. The spectrum data files can be opened in other analysis
softwares.
S430NIR spectrophotometer can be widely used in oil, alcohol,
beverages and other liquid quality of fast nondestructive analysis.
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