Discover Bio
™
Manual Microwave Peptide Synthesizer
600423 • Revision 2 • August 2017
11
General Discover Bio Methods
Swelling the Resin
1. Set both knobs to “Stop”.
2. Turn on the nitrogen source, then turn on the nitrogen to the system by setting the nitrogen control
knob to pressure (the knob should be pointing down) and the vacuum pump.
3. Slide the drain line out of the groove in the top of the vessel assembly. Open the reaction vessel by
pressing the vessel body up and rotating.
Add the resin to the vessel. Seal the vessel by pressing the vessel body up into the vessel top and
rotating. Slide the drain line back into the groove in the top of the vessel assembly. Place the
vessel in the reaction vessel holder.
4. Set the left knob to “Wash Solvent” to begin adding solvent to the reaction vessel.
NOTE
It does not matter which “Stop” position is used on the right knob.
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