Discover Bio
™
Manual Microwave Peptide Synthesizer
600423 • Revision 2 • August 2017
4
Hardware Assembly and Setup
Wash Station Setup
When setting up the wash station, all tubing connections will be made on the rear of the unit. There are
eleven ports on the manifold, as well as two stainless steel ports. The proper connections for the manifold are
summarized in the table below.
Bottle Setup
1. Place the three 1 L glass bottles in the bottle holder.
2. Set up the Wash Solvent bottle.
2.1. Connect a bottle cap to the 1 L bottle to be used for wash solvent. Ensure the dip tube
reaches the bottom of the bottle and that the dip tube lter is in place.
2.2. Connect the bottle tubing to the cap. The tube labeled “LIQ” should be connected to the
same port as the dip tube. The unlabeled tube should be connected to the port without a dip
tube.
2.3. Connect the other end of the bottle tubing to the rear of the Wash Station. The tube labeled
“LIQ” should be connected to position 4. The unlabeled tube should be connected to
position 1.
3. Set up the Deprotection bottle.
3.1. Connect a bottle cap to the 1 L bottle to be used for deprotection solution. Ensure the dip
tube reaches the bottom of the bottle and that the dip tube lter is in place.
3.2. Connect the bottle tubing to the cap. The tube labeled “LIQ” should be connected to the
same port as the dip tube. The unlabeled tube should be connected to the port without a dip
tube.
3.3. Connect the other end of the bottle tubing to the rear of the Wash Station. The tube labeled
“LIQ” should be connected to position 5. The unlabeled tube should be connected to position
2.
Position
Number
Tubing Connection
1 Main Wash Nitrogen
2 Deprotection Nitrogen
3 Waste Vacuum
4 Main Wash Liquid
5 Deprotection Liquid
6 Waste Liquid
7 Reaction Vessel Sprayhead
8 Reaction Vessel Drain
9 Reaction Vessel Vent
10 Product Position Vacuum
11 Product Position Liquid
SS-1 Nitrogen Source
SS-2 System Vent
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