CEM Discover Bio - Recommended Synthesis Parameters; Recommended Microwave Methods; Recommended Reagents for 0.1 Mmol Scale

Discover Bio
™
Manual Microwave Peptide Synthesizer
600423 • Revision 2 • August 2017
9
Recommended Synthesis Parameters
Recommended Microwave Methods
Recommended Reagents for 0.1 mmol Scale
Activator Base Solution
Prepare a solution of ~0.3 M DIEA in DMF.
Deprotection Solution
Prepare a solution of 20% piperidine in DMF with 0.1 M HOBt monohydrate.
Method Power Max Temp. Run Time Delta Temp.
Deprotection 20 W 75 °C 3:00 5 °C
Coupling 20 W 75 °C 5:00 5 °C
TFA Cleavage 15 W 38°C 30:00 5 °C
NOTE
Cysteine and histidine couplings should be performed at 50 °C to reduce racemization. Cleavage
temperatures should never exceed 43 °C. All values for Power are suggested, and should be
adjusted depending on reaction scale. The goal is to reach the maximum temperature in 75
seconds for Deprotection and Coupling, and in 120 seconds for Cleavage.
Number of
Couplings
0.1 mmol Scale
mL
DMF
mL
DIEA
Final
Vol.
5 19 1 20
10 38 2 40
Per Coupling: 4 mL
Final Volume
0.1 mmol Scale
mL
DMF
mL
Pip.
g HOBt
monohydrate
50 40 10 0.77
100 80 20 1.5
500 400 100 7.7
Per Coupling: 7 mL
NOTE
HOBt is optional, but is recommended for sequences susceptible to aspartimide formation.
WARNING
The Discover Bio reaction vessels should not be operated above 75 ºC.