CEM Discover Bio - Performing a Cleavage

Discover Bio
™
Manual Microwave Peptide Synthesizer
600423 • Revision 2 • August 2017
13
3. Draw the coupling solution into a 10 mL syringe. Insert the luer tip of the syringe into the injection
port. Inject the coupling solution through the injection port into the reaction vessel.
4. To purge the injection port line, draw air into the syringe, then inject the air through the injection
port to fully purge the coupling solution into the reaction vessel.
5. Repeat step 4.
6. Set the left knob to “Stop”. Set the right knob to “N2 Bubbling”.
7. Ensure the ber-optic probe is fully inserted into the thermowell.
8. Insert the reaction vessel into the Discover cavity. Load the Coupling microwave method, and press
the Play/Pause key to begin the method.
9. When the microwave method is nished, remove the reaction vessel from the Discover cavity and
place it in the vessel holder. Set the right knob to “Waste” to drain the reaction vessel.
10. When all the solvent has drained, set the right knob to “Stop”. Set the left knob to “Wash Solvent”
and add approximately 7 mL of solvent to the vessel, then set the left knob to “Stop”. Optionally,
to bubble the resin for a more efcient wash, turn the right knob to “N2 Bubbling” and allow the
resin to bubble for ve seconds.
11. Set the left knob to “N2 Purge (Wash)”, then set the right knob to “Waste” to drain the reaction
vessel. Set both knobs to “Stop”.
12. Repeat steps 10 and 11 two more times.
Performing a Cleavage
1. Set both knobs to “Stop.” Turn off the nitrogen source.
2. Remove the solvent bottle, then connect a solvent bottle of DCM.
3. Turn on the nitrogen source.
4. Set the left knob to “Wash Solvent” and add approximately 7 mL of solvent to the vessel, then set
the left knob to “Stop”. Optionally, to bubble the resin for a more efcient wash, turn the right
knob to “N2 Bubbling” and allow the resin to bubble for ve seconds.
5. Set the left knob to “N2 Purge (Wash)”, then set the right knob to “Waste” to drain the reaction
vessel. Set both knobs to “Stop”.
6. Repeat steps 4 and 5 two more times.
NOTE
Purging the line will add an additional 1-2 mL of coupling solution to the reaction vessel.
WARNING
The Discover Bio reaction vessels should not be operated above 75 ºC.