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If the sample contains bound water such as an all-meat emulsion, cooked all-meat
sausage, sausage with extenders, semi-dry sausage, or ham, place the sample on
the end of the spatula and apply the sample to the middle of the pad. Then spread
the sample around the pad in a circle to generate thermal heat.
2 Spread Layer on Pad
If the sample is a heat sensitive sample such as a water-based or solvent-based
liquid, use a pipette to dispense the sample onto the pad(s). Sample size should be
2-5 grams, based on solid content of the sample – 2-3 grams for high solids
samples, 4-5 grams for samples containing less than 15 percent solids. Samples
containing less than two percent solids may require as much as 10 grams for
analysis. Large quantity samples should be pipetted onto the pads in a thin even
layer. Additional glass fiber pads may be used for increased absorbency. A
sufficient number of pads should be used to ensure that liquid samples do not drip
onto the floor of the instrument cavity.
3 Puddle, Spread with Pipette and Cover
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