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CID Bio-Science CI-340 User Manual

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Table of Contents
Introduction ................................................................................................................................................... 1
Infrared Gas Analysis (IRGA) ................................................................................................................ 1
Dispersive and Non-Dispersive IRGA .................................................................................................... 2
Photosynthesis Measurement Using IRGA............................................................................................. 2
Technical Information ................................................................................................................................... 3
Main Components of Gas Exchange Systems ........................................................................................ 4
Highlights of CI-340 ............................................................................................................................... 4
General Operating Instructions ...................................................................................................................... 7
Using the Keypad .................................................................................................................................... 7
File Menu ................................................................................................................................................ 8
Installation of the Battery ........................................................................................................................ 9
Interpretation of Parameters .................................................................................................................. 11
System Setup and Calibration ..................................................................................................................... 12
Calendar and Time Setup ...................................................................................................................... 13
Leaf/Air Temperature Sensor ............................................................................................................... 13
Atmospheric Pressure ........................................................................................................................... 13
Flow Rate .............................................................................................................................................. 14
CO2 and H2O General Overview ......................................................................................................... 15
CO2 Zero and CO2 Span Procedure ..................................................................................................... 17
H2O Zero and H2O Span Procedure .................................................................................................... 18
Data Transfer ............................................................................................................................................... 19
Downloading Data ................................................................................................................................ 20
Updating Software ................................................................................................................................ 21
Example of a Data File ......................................................................................................................... 22
Photosynthesis, Transpiration and Stomatal Conductance .......................................................................... 23
Taking Measurements ........................................................................................................................... 23
Closed and Open Systems ..................................................................................................................... 26
Terminology: ............................................................................................................................................... 28
Absolute, Differential and Continuous Mode ....................................................................................... 28
Photosynthesis ...................................................................................................................................... 28
Photosynthetic Active Radiation (PAR) ............................................................................................... 29
Transpiration ......................................................................................................................................... 29

Table of Contents

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CID Bio-Science CI-340 Specifications

General IconGeneral
ModelCI-340
DetectorSilicon Photodiode
GPSIntegrated GPS
Light SourceLED
Resolution (SPAD)0.1 SPAD unit
Accuracy±1 SPAD unit
Data StorageInternal memory, expandable with SD card
DisplayLCD screen
Operating Temperature0 to 50°C
Measurement TypeChlorophyll Content (SPAD)
Humidity Range0-85% relative humidity, non-condensing

Summary

Introduction to the CI-340 System

Infrared Gas Analysis (IRGA)

Explains the principle of infrared gas analysis for measuring gases like CO2 and H2O.

Photosynthesis Measurement Using IRGA

Details how IRGA is used to measure photosynthesis rates and related plant parameters.

Technical Information

System Overview and Airflow

Describes the CI-340's technical components, airflow path, and measurement process.

Main Components of Gas Exchange Systems

Lists and explains the primary components of the CI-340 gas exchange system.

Highlights of CI-340

Summarizes key features and advantages of the CI-340 photosynthesis system.

General Operating Instructions

Startup and Acclimation Procedures

Details the necessary warm-up and acclimation procedures before taking measurements.

Using the Keypad

Explains the function and usage of the CI-340's keypad for navigation and input.

File Menu Operations

Describes how to access and manage data files stored within the CI-340's file system.

Installation of the Battery

Interpretation of Parameters

System Setup and Calibration

Calendar and Time Setup

Guides the user on how to set the instrument's date and time for accurate record-keeping.

Leaf/Air Temperature Sensor Calibration

Explains the calibration process for the leaf and air temperature sensor.

Atmospheric Pressure Calibration

Details how to calibrate the atmospheric pressure sensor for accurate readings.

Flow Rate Calibration

CO2 and H2O General Overview

CO2 Zero and CO2 Span Procedure

Step-by-step guide for calibrating the CO2 zero and span settings.

H2O Zero and H2O Span Procedure

Step-by-step guide for calibrating the H2O zero and span settings.

Data Transfer

Downloading Data

Instructions on how to transfer collected data from the CI-340 to a computer.

Updating Software

Explains the process for updating the CI-340's internal software code.

Example of a Data File

Photosynthesis, Transpiration and Stomatal Conductance

Taking Measurements

Guides the user through the process of initiating and conducting measurements with the CI-340.

Terminology

Absolute, Differential and Continuous Mode

Explains the different measurement modes available on the CI-340.

Photosynthesis Definition

Defines photosynthesis in the context of plant physiology.

Photosynthetic Active Radiation (PAR)

Defines PAR and its measurement by the CI-340 system.

Transpiration

Explains transpiration and its determination using the CI-340.

Stomatal Conductance

Open and Closed Systems

Measuring Conifer Needles Using the CI-340

LC-4 Small Cylinder Chamber Specifications

Provides specifications for the LC-4 small cylinder chamber used with CI-340.

LC-5 Large Cylinder Chamber Specifications

Provides specifications for the LC-5 large cylinder chamber used with CI-340.

Estimating Leaf Area

Calibrating

DATA DISPLAY SCREENS

GRAPH MODE

CARE OF THE CI-340

Leaf Chamber Care and Use

Details proper handling, attachment, and care for CI-340 leaf chambers.

RECHARGING

Powering from Lab Socket

Explains how to connect the CI-340 to an external AC power source.

Disconnecting Charger/Adapter

Instructions for safely disconnecting the battery charger and adapter.

Charging the Li-ion Battery

Step-by-step instructions for charging the CI-340's Li-ion battery.

EQUATIONS

TROUBLESHOOTING (General)

Display Issues

Addresses common problems related to the instrument's display not turning on or flickering.

Keypad Not Responding

Provides solutions for when the CI-340's keypad keys do not respond correctly.

Reading Fluctuations

Offers solutions for fluctuating or deteriorating CO2/H2O readings during measurements.

SYSTEM SPECIFICATIONS

CO2 Analyzer Specifications

Details the technical specifications for the CI-340's CO2 analyzer.

H2O Analyzer Specifications

Details the technical specifications for the CI-340's H2O analyzer.

PAR Sensor Specifications

Lists the technical specifications for the Photosynthetically Active Radiation (PAR) sensor.

Temperature Measurement Specifications

Covers specifications for chamber and leaf temperature measurement sensors.

POWER PACK

ACCESSORY CONTROL PORT AND CABLE

TROUBLESHOOTING (Support and FAQs)

Technical Support

Information on how to obtain technical assistance and support for the CI-340.

Customer Service

Details on how to contact customer service for sales, upgrades, or repairs.

FAQs

Directs users to the FAQ section for common questions about the CI-340.

APPENDIX A: CI-510CS TEMPERATURE CONTROL MODULE

CI-510CS Temperature Control Module Overview

Introduces the CI-510CS module for controlling leaf and air temperature.

Filling the Reservoir

Instructions for filling the water reservoir for the CI-510CS temperature control module.

Emptying the Reservoir

Procedures for draining the water reservoir after using the CI-510CS module.

Operating the Temperature Control Module

Guides on how to operate the CI-510CS module for temperature control.

APPENDIX B: CI-301LA LIGHT ATTACHMENT

CI-301LA Light Attachment Overview

Introduces the CI-301LA module for providing controlled light for photosynthesis measurements.

Connecting and Operating the Light Attachment

Instructions for connecting and using the CI-301LA light attachment with the CI-340.

APPENDIX C: CI-301AD ADJUSTABLE H2O AND CO2 CONTROL MODULE

CI-301AD Module General Description

Provides a general overview of the CI-301AD module for controlling CO2 and humidity.

Connecting to Power and Analyzer

Details how to connect the CI-301AD module to power and the CI-340 analyzer.

Operation of the CI-301AD Module

Explains the basic operation and handling of the CI-301AD module.

Replacing Consumables

Guides on replacing consumables like soda lime, silica gel, and filters for the CI-301AD.

APPENDIX D: CI-301SR SOIL RESPIRATION CHAMBER

CI-301SR Soil Respiration Chamber

Introduces the CI-301SR module for measuring soil respiration.

Setup Procedures for Soil Respiration

Outlines the steps for setting up and performing soil respiration measurements with the CI-301SR.

APPENDIX E: CANOPY CHAMBER ASSEMBLY INSTRUCTIONS

Canopy Chamber Assembly Instructions

Provides instructions for assembling canopy chamber attachments for the CI-340.

Flexible Chamber Wall Assembly

Details how to assemble canopy chambers using flexible materials.

Rigid Chamber Wall and Attachment

Explains assembly for rigid canopy chambers and attaching them to the CI-340.

How to Measure with Canopy Chamber

Guides on performing measurements using the canopy chamber attachment.

APPENDIX F: CI-510CF CHLOROPHYLL FLUORESCENCE MODULE

CI-510CF Module Overview

Introduces the CI-510CF module for chlorophyll fluorescence measurements.

Connecting CI-510CF to CI-340

Instructions for connecting the CI-510CF module to the CI-340 instrument.

Fluorescence Saturation Pulse Measurement

Details how to perform fluorescence saturation pulse measurements using the CI-510CF.

Warranty Information

Seller's Warranty and Liability

Defines CID Bio-Science's warranty coverage and limitations for its manufactured equipment.