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DAKEWE 6250 - User Manual

DAKEWE 6250
47 pages
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Revised Version V4.1-English Version-June 2021
Dakewe(Shenzhen)Medical Equipment Co,. Ltd 1 V4.1
Cryostat
Users Manual
Applicable Model:
6250

Table of Contents

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Summary

Information

Symbol and Meaning

Explains the meaning of various symbols used in the manual for safety and understanding.

Instrument Use

Details the intended use, components, and limitations of the cryostat instrument.

Working Principle

Describes the fundamental mechanism by which the cryostat operates to prepare tissue slices.

Personnel Qualification

Specifies the required training and qualifications for operating the instrument safely and effectively.

Instrument Model

Provides information on the specific model and software versions of the cryostat.

Contraindications

Lists any conditions or situations where the instrument should not be used.

Safety

Safety Precautions

Outlines essential safety guidelines and procedures for operating and maintaining the cryostat.

Warning - Signs on the Instrument

Details specific warning labels found on the instrument and their significance for user safety.

Warning - Transport and Installation

Provides critical warnings related to the safe handling, transportation, and installation of the cryostat.

Warning - Operation

Lists crucial safety instructions and warnings to follow during the operation of the cryostat.

Warning - Cleaning and Maintenance

Highlights essential safety warnings to be observed during cleaning and maintenance procedures.

Equipment Placement Requirements

Specifies the environmental and physical conditions required for optimal and safe instrument placement.

Instrument Handling

Describes the correct procedures for safely moving and positioning the cryostat instrument.

Precautions for Use of the Instrument

Encompasses vital precautions to ensure safe and proper usage of the cryostat after installation.

Instrument Components and Specifications

Overview

Provides a visual introduction to the cryostat's main parts and their identification.

Technical Parameters

Details the technical specifications, performance characteristics, and operational ranges of the cryostat.

First Installation

Installation Requirements

Outlines the necessary environmental and site conditions for installing the cryostat.

Unpacking Tools

Lists the tools recommended for safely unpacking the cryostat from its packaging.

Unpacking

Guides users through the step-by-step process of safely removing the cryostat from its shipping container.

Accessory Installation

Covers the procedures for installing various accessories essential for the cryostat's operation.

Hand Wheel Installation

Details the correct method for installing the hand wheel component onto the cryostat.

Hand Wheel Block Installation

Explains the process of installing the hand wheel block accessory for proper functionality.

Unlock the Instrument Cover

Describes how to unlock and open the instrument's cover for access or maintenance.

Fixed Card Core

Details the procedure for securing the fixed card core to prevent damage during transport.

Waste Trough Installation

Guides on how to properly install the waste trough for collecting discarded materials.

Blade Holder Installation

Provides instructions on how to install the blade holder assembly for sectioning.

Glass Door Installation

Explains the steps for correctly installing the glass door onto the cryostat chamber.

Power Supply Cable Connection

Outlines the procedure for connecting the cryostat to the power supply.

Boot Preparation

Details the essential steps to take before powering on the cryostat for the first time.

Operation

Section Function Setting

Configures various settings related to the sectioning process, including temperature and slicing parameters.

Cooling Temperature Setting

Guides users on setting and adjusting the operating temperature for the cryostat's cooling chamber.

Slice and Retouch Setting

Covers the settings for controlling slice thickness and retouching parameters during operation.

Cold Stage Temperature Setting; Semiconductor Cooling Function

Details how to manage cold stage temperatures and utilize the semiconductor cooling feature.

Lighting and UV Lamp

Explains the function and operation of the internal lighting and UV disinfection lamp.

Drying Button

Describes the process and precautions for using the drying function of the cryostat.

Button Panel

Identifies and explains the function of each button on the cryostat's control panel.

Function Setting and Introduction

Introduces and explains various functional settings available on the cryostat interface.

Defrost Setting

Details how to configure manual and automatic defrost cycles for the cryostat.

Hibernation Setting

Explains how to set up hibernation mode for the cryostat, including timing and temperature.

Disinfection Setting

Covers the procedures for manual and automatic disinfection cycles of the cryostat.

Time reset in the function interface

Describes how to reset date and time settings to factory defaults.

Compressor Overheat Protection Function

Explains the built-in safety feature that protects the compressor from overheating.

System Setting

Allows for configuration of system-wide parameters, including time and retraction values.

System Time Setting

Details how to set and synchronize the system's internal clock.

Slice Retraction Value Setting

Explains how to adjust the slice retraction value for optimal sectioning.

Blade Holder Module

Provides a detailed description of the blade holder assembly and its components.

Blade Holder Front and Rear Position Adjustment

Guides on adjusting the front and rear position of the blade holder for precise sectioning.

Blade Holder Left and Right Position Adjustment

Explains how to adjust the left and right position of the blade holder.

Blade Installation or Replacement

Covers the safe and correct procedure for installing or replacing the microtome blade.

Anti-roll Plate Adjustment

Details how to adjust the anti-roll plate for optimal tissue slice quality.

Microtome Blade Angle Adjustment

Explains the process of adjusting the microtome blade angle for precise cutting.

Sample disk Installation

Guides on how to properly install the sample disk for specimen placement.

Specimen Cryoforming

Describes the process of preparing and freezing specimens on the cryostat shelf.

Sectioning Operation

Starting the Instrument

Outlines the steps required to safely power on and start the cryostat.

Preparing Sectioning Tools, Specimen Disc and Auxiliary Preparation Tools

Lists the necessary tools and materials to prepare before commencing sectioning.

Retouching Slice

Explains the preliminary stage of retouching slices before final sectioning.

Sectioning

Details the core process of performing tissue sectioning using the cryostat.

Sectioning Reference

Provides tips and references for achieving optimal sectioning quality and technique.

Cleaning and Maintenance

Daily finishing work

Describes the routine tasks to perform at the end of each day for instrument upkeep.

Long-term Suspension of Finishing Work

Outlines procedures for preparing the instrument for extended periods of non-use.

Cleaning Instrument

Provides general guidelines and restrictions for cleaning the cryostat.

Before each cleanup, perform the following steps first

Lists preparatory steps to take before initiating any cleaning process.

Cleaning the Instrument Enclosure

Details the method for cleaning the exterior surfaces of the cryostat.

Cleaning the Blade Holder

Explains the specific procedure for cleaning the critical blade holder components.

General Maintenance

Covers regular maintenance tasks to ensure the cryostat's performance and longevity.

Take the Slicing Core from the Freezer

Details the procedure for safely removing the slicing core for maintenance or repair.

Replacing Component

Provides instructions for replacing common components such as fuses and UV lamps.

Replacing the Fuse

Guides on how to safely replace a blown fuse in the cryostat.

Replacing the UV Lamp

Explains the procedure for replacing the UV lamp used for disinfection.

Use of Waste Tank

Describes the proper usage and maintenance of the waste liquid collection tank.

FAQ and Handling

Section Tissue Temperature Selection Table

Warranty and Service

Warranty

Outlines the terms and conditions of the product warranty provided by the manufacturer.

Technical Service Information

Provides contact details and information required for requesting technical support or service.

Scrapping and Disposal

Gives guidance on the proper and compliant methods for disposing of the instrument at the end of its life.

DAKEWE 6250 Specifications

General IconGeneral
Model6250
Accuracy±5%
TypeInfusion Pump
Power SupplyAC 100-240V, 50/60Hz
DisplayLCD
AlarmsOcclusion, Low battery