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Esco Class II - 6.2. Recommended Decontamination Sealing Method

Esco Class II
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32
The BIOQUELL principle is to seek micro-condensation to achieve the kill. The generator releases tiny
high-speed droplets inside the BSC.
Hydrogen peroxide vapor is non-carcinogenic, but highly effective against micro-organisms. Hydrogen peroxide
(H
2
O
2
) vapor breaks down under catalytic action to become air and water, making it environmentally friendly
and it leaves no residues. The BSC is often aerated by ducting to speed up the breaking down of hydrogen
peroxide vapor.
The time needed for the entire process is outlined below:
No.
Process
Time
1
Set
-
up & sealing the BSC
½ hour
2
Conditioning and decontamination cycle
½
-
1 ½ hour
3 Ducting out H
2
O
2
H
2
O
2
generator doing aeration ½ hour 8 hours
4 Tear-down ½ hour
TOTAL 2 - 3 hrs 9½ - 10½ hrs
For hydrogen peroxide decontamination, the BSC need to be equipped with two ports:
1. One port located in front opening or side wall, penetrating the work zone area
2. One port located on top of the exhaust filter.
The generator used defines the port function as described below:
Steris Bioquell
Hydrogen peroxide source Injected into the BSC Generated inside the BSC
Bottom front / side port Hydrogen peroxide introduction Hydrogen peroxide re-introduction
Top port Hydrogen peroxide extraction Hydrogen peroxide extraction
6.2. Recommended Decontamination Sealing Method
This section gives an example of how the cabinet can be sealed when FV-011 formalin vaporizer is used.
However, similar principle can be applied for other gaseous decontamination method.
1. Put the decontamination bag to enclose the
entire BSC and support stand.
2. Use the 50 mm wide aluminum tape to seal the
gap between the decontamination bag and the
floor. To compensate for areas where the
decontamination bag must turn, use multi section
tape. Ensure that the two layers of tape overlap
at the end points to prevent leakage.

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