Forma Scientific, Inc.__________________________________________Installation/Start-up
3-9
Turn the water off immediately when the ADD WATER alarm light goes out.
Using the funnel, add an additional quart (one liter) of distilled water.
Remove vinyl tubing and reinstall the plastic protective cap.
3.11 Filling the Humidity Reservoir or Pan
Do not use plastic pans for humidification as they will have an unpredictable effect on
humidity and O
2
/CO
2
levels in the incubator. Use only the floor of the unit or the
optional stainless steel humidity pan.
Do not use de mineralized or de ionized water in the humidity reservoir or pan unless
it has been boiled immediately prior to use as it may be contaminated with bacteria.
Listed below are two recommended methods of providing elevated humidity in the
chamber.
Note: Frequent door openings will cause humidity loss from the chamber. Recovery time will
also be affected.
1. Reservoir
The reservoir in the bottom of the incubator may be filled with at least 3/4" of sterile
distilled (50K ohm to 1 Meg ohm) water.
2. Humidity Pan
The optional humidity pan (Forma Stock #237001) will hold about 6 quarts (5.8 liters)
of sterile distilled (50K ohm to 1 Meg ohm) water. Note: This pan may be autoclaved.
The best humidity and temperature response from the humidity pan is obtained when the
pan is placed directly on the incubator floor.
The water level in the humidity reservoir should be checked frequently. If a
disinfectant has been added to the water in the reservoir, it should be changed once a
week to help prevent microbial contamination. If a disinfectant has not been added to the
sterile distilled water, the water should be changed at least twice a week.
It is important that the water level in the reservoir or pan be kept relatively constant
as fluctuations or "dry-outs" will have an adverse effect on the humidity level and CO
2
control in the chamber.
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