Forma Scientific, Inc.__________________________________________________Operation
4-1
Section 4 - Operation
4.1 Operation
The water jacket is filled with 11.5 gallons (43.5 liters) of water through the fill fitting
located on the front of the unit. The water is then warmed by the chamber heater,
providing very stable heating of the incubator chamber. The water stays at a constant
temperature with a minimum of heater on-time and also acts as insulation from ambient
temperatures.
Temperature control is maintained by a proportional, zero-switching device which
provides temperature uniformity throughout the chamber. A separate and independent
over temperature controller assures product safety by assuming control at the over temp
setpoint if the primary controller fails. Should an over temp condition develop, the
monitor alarm system will alert the operator that a malfunction has occurred.
An internal blower gently circulates the air in the chamber to prevent CO
2
stratification and minimize culture desiccation.
4.2 Overview of Humidification and CO
2
Thermal conductivity of the air inside of the incubator is affected not only by the
quantity of CO
2
present, but by the quantity of water vapor present in the incubator
atmosphere. This effect is linearly related to the absolute humidity of the atmosphere (See
figure below).
Moisture Effect on CO
2
Control Calibration
Figure 4-1
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