Prerequisites
● You have added the LV Pick Tip command to the Task List.
To set aspirate and dispense commands:
1. Drag and drop the appropriate plate type you are using from the Plate Type library
(bottom of screen) to the microplate station on the screen.
2. Optional: If you want to add commands for every well on a plate, click on
the Task List.
3. Double-click the microplate. The Task Details dialog appears.
4. Use the table below as a guide to using the Task Details dialog box.
Note: You must use station 1 if you are dispensing LCP drops.
Repeat
Note: The microplate Task Details information and Select Target image may vary depending
on the microplate type in use.
Area Description Information
Action Provides two options: Aspirate and Dispense.
Black Arrow Specifies the direction of a specific Action. Normal direction
(column 1 to column 12) is indicated by the right-arrow. To
reverse the direction, click it.
Column Represents the well column numbers. Select the desired well
column number by clicking on the corresponding number.
For multiple selection, hold the SHIFT key and click the
desired well column number. If a column is selected, it
appears in dark blue. The NT8 will perform the action on the
selected number.
Liquid Class Provides four liquid class options: Normal (N), Medium (M),
High (H), and Very High (V). It's important to select the
correct liquid class for each liquid, and on a column-by-
column basis when necessary.
Note: You can apply liquid class to individual well columns if
the Repeat command on the Task List is selected.
Volume Set the liquid volume to be aspirated or dispensed by typing
a value into the Volume field. You can also click the up or
down arrows to increase or decrease the volume.
Mixing (LCP only) Determines the number of times you want the NT8 to mix the
LCP sample and well solution before dispensing.
Z-offset Enables you to change the Z-offset value. The Z-offset value
is the clearance between the tips or LCP tip and plate surface.
Note that if the flexible fingers ( ) are active, the system will
automatically adjust the Z-offset (dispense only).
NT8 User's Guide | Chapter 8: Software Overview
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