NT8 User's Guide | Chapter 8: Software Overview
Important: Please make sure that you set the volume with the actual volume available
in each reservoir. You must have the same volume in each reservoir across a column.
The column number should match the actual column number as the protein source
that has been filled with protein.
Option Description
Aspirate/Dispense
● Action: Provides two options: Aspirate and Dispense.
Select Aspirate if you want the NT8 to aspirate protein
from the protein source.
● Column: Enables you to select the protein source column
number. The NT8 will perform the action on the selected
column. Please note that the protein column numbers
will be disabled if you haven't set the protein volume (see
Setting the Protein Volume).
● Volume: Set the protein volume to be aspirated or
dispensed by typing a new value into the corresponding
field, or clicking the up and down-arrows to increase or
decrease the volume.
● Liquid Class: Enables you to select the protein liquid
class.
THE PROTEIN SOURCE STATION
The protein source station is an area in the software where you interact with your protein
source. Here you define the initial protein source volume and the protein liquid class. These
settings are needed for sitting drop, hanging drop, seeding and serial dilution experiments.
Prerequisites
● You know if you are using a HV Protein Block or LV Protein Block.
To set the protein volume:
1. If you are using a LV protein block, right-click the protein source image and select Set
Protein Volume. If you are using a HV protein block, select Set High Volume
Protein Tray. The Protein Volume Setup dialog appears
2. Leave the box next to Auto checked. If it is unchecked, the NT8 will not automatically
use the reagent liquid class as defined in the Task Details dialog box.
3. Fill in the volume available in each reservoir of a particular column. If you are using a
high volume protein block, you can specify a volume of up to 13 µL.
4. Click OK.
Protein Source Station Right-Click Menu Options
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