Gilson ASPEC - General Flow Guidelines

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ASPEC® POSITIVE PRESSURE MANIFOLD | USER'S GUIDE
OPERATION
General Flow Guidelines
The following table provides general process and flow guidelines.
Table 1
SPE Process and Control Guidelines for SPE Steps
SPE Steps
Process
Control
Setting
SPE Control Setting
NOTE
For all low and high flow recommendations, refer to the
SPEFormat Flow Guidelines table below.
Condition SPE High Flow, and then switch to LowFlow
Load SPE Low Flow, based on viscosity
Wash SPE High Flow, based on viscosity
Dry Dry O
Elute SPE Low Flow, based on viscosity
Table 2
SPE Format Flow Guidelines
SPE Format
High Flow
(drops per second)
Low Flow
(drops per second)
6 mL 3 to 5 1
3 mL 1 1 drop per 2 to 3 seconds
1 mL
microplate
0.5 to 1 1 drop per 3 to 4 seconds