Gilson ASPEC - Basic SPE Technique

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USER’S GUIDE | ASPEC™ POSITIVE PRESSURE MANIFOLD
OPERATION
Basic SPE Technique
1. Ensure that the necessary conditions for
operation are met. Refer to page 22.
2. Follow the instructions to
Setup for Flow to Waste on page 22.
Initial Condition Step
3. Pipette the appropriate volume of
conditioning solvent into each available
SPE cartridge.
4. Seat the manifold. Refer to
Seat the Manifold on page 24.
5. Switch the SPE control dial to High Flow.
6. Turn the high flow adjustment knob
clockwise until the desired flow rate is
achieved.
7. When the solvent levels decrease to
2mm above the fastest eluting cartridge
of any one of the SPE cartridge/s, quickly
switch the SPE control dial to Low Flow.
8. Turn the low flow adjustment knob clockwise until the flow slowly
restarts.
9. When the solvent levels lower to the top frits of all of the SPE cartridges,
quickly switch the SPE control dial to Off. Allow the solvent to return to
atmospheric pressure.
NOTE
In some cases, positive pressure can remain in the
SPEcartridges, even after the SPE control dial has been set
to O. Unseat the manifold to stop unwanted flow.
Refer to page 24.
Figure 22
SPE Cartridge Diagram
SPE Sorbent
Top Frit
Bottom Frit