Gilson ASPEC - Troubleshooting Table

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ASPEC® POSITIVE PRESSURE MANIFOLD | User's GUideUSER’S GUIDE | ASPEC™ POSITIVE PRESSURE MANIFOLD
TroUbleshooTinG Table
TroUbleshooTinG Table
Troubleshooting Table
Symptoms Possible Causes SPE Steps Solutions
Lack of flow
No inlet pressure.
All
Check that the gas pressure source is set to 15 psi.
O-ring damaged or missing. Replace the O-ring. Refer to page 31.
Damaged process control dial.
Replace the front panel. Contact your local Gilson representative. Refer to
page 36.
Liquid flows unevenly
Channeling inside the sorbent
of the SPE cartridge/s.
Condition
Specific Causes Specific Solutions
Drying of the cartridge/s. Repeat the condition step.
Insucient condition volume. Increase the condition volume.
Excessive flow rate. Reduce the flow rate.
Clogged SPE cartridge/s.
Condition Replace the SPE cartridge/s.
Load
Reconsider the sample preparation workflow; e.g., sample filtration,
centrifugation, etc.
Heterogeneous viscosity
inside the SPE cartridge/s,
caused by the use of
dierent liquids in successive
SPEsteps.
Condition
Reduce the residual level of liquid above the frit to 1 to 2mm before
conditioning with another type of liquid.
Wash and
Elute
Before using a new solvent, ensure that all euent has been expelled from
the SPE cartridges.
Leak in the manifold gasket. All Replace the manifold gasket. Refer to page 31.
Liquid continues to flow,
even after the SPE control
dial has been set to O.
The process control dial is set
to Dry.
All
Switch the process control dial to SPE.
Residual positive
pressure remains in the
SPEcartridge/s.
Switch the SPE control dial to Low Flow before stopping. Unseat the
manifold. Refer to page 24.