Gilson ASPEC - Page 29

29
ASPEC® POSITIVE PRESSURE MANIFOLD | USER'S GUIDE
OPERATION
Additional Condition Steps
10. Unseat the manifold. Refer to page 24.
11. Repeat steps 3 through 9 for the appropriate number of conditioning
steps.
NOTE
If using an identical solvent for subsequent conditioning
steps, use the flow adjustment locks to ensure the same
pressure values are used.
Sample Load Steps
12. Pipette the appropriate volume of sample into each available
SPEcartridge.
13. Seat the manifold. Refer to page 24.
14. Switch the SPE control dial to Low Flow.
15. Turn the low flow adjustment knob clockwise until the desired flow rate
is achieved.
16. When the sample stops dripping from the SPE cartridges, turn the
SPEcontrol dial to Off.
17. Unseat the manifold. Refer to page 24.
Wash Steps
18. Pipette the appropriate volume of washing solvent into each available
SPE cartridge.
19. Seat the manifold. Refer to page 24.
20. Switch the SPE control dial to High Flow.
21. Turn the high flow adjustment knob clockwise until the desired flow rate
is achieved.
22. When the washing solvent stops dripping from the SPE cartridges, turn
the SPE control dial to Off.
23. Unseat the manifold. Refer to page 24.
24. Repeat steps 18 through 23 for the appropriate number of washing
steps.