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SURFACTANTS, ANIONIC
Note: Chloroform is more dense than water will sink to the bottom
of the graduated glass vial.
• Invert the vial twice and remove the cap to release any pressure that
has built up.
• Close the glass vial with its cap and shake it vigorously for 30 seconds.
Note: Ensure the cap is secure when shaking.
•Press Timer and the display will show the countdown or, alternatively, wait for 2 minutes.
During this period the chloroform layer separates from the aqueous layer, the color of the
aqueous layer will fade slightly, while the chloroform layer will turn blue.
• Remove the cap.
• Remove the upper aqueous layer using the long plastic pipette, do not
remove the lower chloroform layer.
• Add 15 mL of deionized water to the vial (up to the 25 mL mark).
• Add 2 drops of HI95769A‑0 Anionic Surfactants Reagent A.
• Invert the vial twice and remove the cap to release any pressure that
has built up.
• Close the glass vial with its cap and shake it vigorously for 30 seconds.
Note: Ensure the cap is secure when shaking.
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