Hitachi PRIMAIDE 1440 - Description of Fluorometry; Principle of Fluorometry

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2.3 Description of Fluorometry
2.3.1 Principle of fluorometry
Fig. 2-4 Typical Organic Molecular Energy Level Diagram
Figure 3-4 shows the energy level transitions of an organic
molecule for the various processes involved in absorption,
fluorescence and phosphorescence.
Radiationless
transition
Radiationless
transition
Absorption
Excitation light
Excited triplet
state
Excited state V =
Phosphorescence
Fluorescence
Ground state V =
3
0
1
2
3
0
1
2
Light
Light
Excitation
Stable condition
(Ground state)
Fluorescence or
Phosphorescence
Unstable condition
(Excited state)