5.6.6 Single/Three-Channel Fluorimeter
The innovative Single/Three-Channel Fluorimeter makes available to the marine,
freshwater, surveying markets a high performance sensor providing the user with increased
functionality when compared to other standard fluorimeters. The Single/Three-Channel
Fluorimeter can be tailored to measure a wide range of fluorescent parameters (see the
below table). Moreover, it is possible to extend the measurement range through the use of
dynamic range function. Dynamic range can be adjusted simply by varying the LED
intensity, while still maintaining the factory set calibration via the internal referencing of
LED output
All versions of the Three-Channel Fluorimeter come with a Chlorophyll a channel as
standard; two other channels can then be selected from phycoerythrin, phycocyanin or
nephelometer (turbidity) options.
The Three-Channel Fluorimeter allows the user to assess the relative contribution to Chlorophyll a
fluorescence emission from the different light harvesting pigments absorbing light at each of the chosen
excitation wavelengths. This information can then be used to make an assessment of the different classes
of phytoplankton present in the sample under analysis.
Features
➢ Range of wavelengths available.
➢ High rejection of ambient daylight.
➢ Low noise high sensitivity.
➢ Low turbidity breakthrough.
➢ Low power consumption.
➢ Rugged corrosion free “Acetal C” housing.
➢ Internal referencing of excitation intensity.
Applications
➢ In Situ Chlorophyll & Algal class study.
➢ Environmental monitoring.
➢ Particulate studies.
➢ River and Stream monitoring.
➢ Pollution monitoring.
➢ Water and Wastewater quality
Fluorescent Parameter Dynamic Range
(*)
Limit of detection
Chlorophyll a 0..100 µg/l < 0.01 µg/l
Fluorescein 0..100 µg/l < 0.005 µg/l
Rhodamine WT 0..100 µg/l < 0.02 µg/l
Phycoerythrin 0..100 µg/l < 0.02 µg/l
Phycocyanin 0..100 µg/l < 0.01 µg/l
Nephelometer (Turbidity) 0..100 FTU < 0.02 FTU
(*) User configurable up to 500 µg/Lor 500 FTU
Note
In case the Single/Three-Channel Fluorimeter is installed in an OCEAN SEVEN 310 CTD which carries out
monitoring by means of the “TIMED DATA ACQUISITION” cycle, it is mandatory to set up the “Number
of data sets per acquisition” up to 10.
5.6.6.1 Fluorimeter special calibration procedure
The Single/Three-Channel Fluorimeter can be re-calibrated by the user using the procedure described
in the dedicated Fluorimeter Operator’s Manual. The calibration is done by using the dedicated interface
software. Therefore, before starting the calibration operations, it is mandatory to install the software in
a personal computer.
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