Qubit
™
4 Fluorometer User Guide
21
3. If you have already performed a calibration for
the selected assay, the software prompts you to
choose between reading new standards and
running samples using the previous
calibration. Press Read standards.
Note: To apply the previous calibration to your sample readings,
press Run samples. The software prompts you to insert the assay
tube containing sample. See “Read samples”, page 24.
4.
At the prompt, insert Standard #1 into the sample chamber and press Read
standard. Be sure to use the Standard #1 appropriate for the assay you are
performing. The reading takes approximately 3 seconds.
5. At the prompt, insert Standard #2 into the
sample chamber and press Read standard.
Be sure to use the Standard #2 appropriate for
the assay you are performing.
6. For Qubit
™
protein and RNA IQ assays only: At
the prompt, insert Standard #3 into the sample
chamber and press Read standard.
7. The calibration is complete after Standard #2 is
read (or after Standard #3 is read for the
Qubit
™
protein or RNA IQ assay). The
software displays the results.
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