22
Qubit
™
4 Fluorometer User Guide
For Qubit
™
quantitation assays: If the calibration is successful, the software
displays the Read standard screen with the Fluorescence vs. Concentration graph.
In the Fluorescence vs. Concentration graph, the standard data points are
connected by a line and open circles represent correct standards.
• For the RNA IQ assay: If the calibration is
successful, the software displays the
Ready for samples message.
• If the calibration is not successful, the software displays the Calibration error
Note: The Read standard screen displays the raw fluorescence values for
Standard #1 and Standard #2 (and Standard #3, if applicable). These values
can assist you in making a judgment regarding the calibration results.
For the Qubit
™
dsDNA BR, Qubit
™
dsDNA HS, Qubit
™
ssDNA, Qubit
™
microRNA, Qubit
™
RNA HS, Qubit
™
RNA BR, Qubit
™
RNA XR, and
Qubit
™
protein assays, the reading given by Standard #2 should be at least
ten times higher than that of Standard #1.
For the Qubit
™
protein assay, the reading given by Standard #3 should be
at least 40% higher than that of Standard #2.
For the Qubit
™
RNA IQ assay, the instrument verifies the calibration
parameters at each stage of the three point calibration process, confirming
the appropriate response of the assay to the standards provided in the kit.
If any issues arise during calibration, the instrument will guide you to
appropriate actions to correct the issue.
To Next Page
Loading...
