Leica M Series User Manual Quick Start Guide 74
The iris diaphragm in the optics carrier of your
M series microscope fulfills the same purpose
as the iris diaphragm in a camera: it regulates
the available light, which changes the depth
of field. The "depth of field" (or "focus depth")
is the area of a specimen that is brought into
sharp focus.
Closing the iris diaphragm
O Close the iris diaphragm by turning the
knob to the left. The subject appears darker
and the depth of field increases.
Opening the Iris Diaphragm
O Open the iris diaphragm by turning the
knob to the right. The subject now appears
brighter, but the depth of field decreases.
Iris Diaphragm
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