microTec
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6. Operation
6.1 Preparation, Orientation, and Alignment of
the Specimen
Prior to starting to cut the specimen, the specimen must be
suitably prepared and embedded and encapsulated in an
appropriate medium. Various molds and plastic cassettes are
used for encapsulation purposes.
By using the microTec adapter system, the specimen can be
aligned to the plane of cut.
6.2 Commencing the Cutting Operation
Macro positioning of the specimen in relation to the knife cutter
is accomplished by moving the base of the knife holder, or by
operating the hand wheel of the coarse feed drive (Fig. 4.11).
With continued operation, cutting of the
specimen commences up to the required depth
of slice. The residues of the cut specimen so produced must be
wiped away from the knife in an upwards direction from
underneath using a brush.
Important!
Do not touch the knife with your fingers!
6.3 Instructions for Producing Sliced Microtomic
Sections of the Specimen
The following aspects are to be heeded in order to produce
effective sliced microtomic sections of the specimen:
• always use a sharp knife,
• as wear occurs at the point of cut, slide the knife to the left or
to the right, in order to be able to continue working with a
sharp section of the knife,
• Always keep the front and rear face of the knife clean. It is
important to thoroughly remove any traces of paraffin wax.