17Stirred Ultraltration Cells
Operating Guidelines
See membrane instructions for cleaning and storage ■
procedures, and for buffer and solvent compat-
ibility.
Highly viscous solutions lter slowly, as do solutions ■
containing particulate matter, such as colloids.
Where a viscous agent (sucrose, glycerin, etc.)
is to be removed, ow can often be increased by
pre-dilution.
Prelter or centrifuge any solution containing par- ■
ticulate matter, such as cell debris or precipitates.
During extended dialtration, liquid level may ■
increase slightly. To correct, concentrate briey.
To maximize recovery of retained substances, ■
continue stirring for a few minutes after
depressurization. This will resuspend the polarized
layer at the membrane surface.
CAUTION: Always check that the pressure-relief
valve knob is set to the horizontal (open)
position, before removing the cell top.
Removal of the top with the pressure-
relief valve knob in the vertical (closed)
position can create a partial vacuum,
which can rupture the membrane.
99228_RevM.indd 17 8/7/2008 3:10:18 PM
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