Labscale Tangential Flow Filtration System User Guide 31
Flushing
1. Disconnect retentate silicone (translucent) tubing from RET
IN port and place end of retentate tubing in waste collection
vessel.
2. Place end of permeate silicone (translucent) tubing into waste
collection vessel. Open retentate valve—turn counterclockwise
.
3. Remove reservoir cover and fill reservoir with 500 mL of
purified water. Remove plug from VENT port and open tank
outlet valve.
PERMEATE TUBING
RETENTATE TUBING
Figure 30: Flushing the Pellicon XL Device, steps 1–6.
4. Turn pump on - increase pump speed until feed pressure
gauge reads 1.38 Bar (20 psi).
Note: Check system connections for leaks—tighten connec-
tions if required
5. Continue pumping to the waste collection vessel until level in
the reservoir drops to 350 mL. Turn pump off.