Labscale Tangential Flow Filtration System User Guide 27
6. Continue pumping the cleaning solution to the waste
collection vessel until the level in the reservoir drops to 250
mL. Turn pump off.
7. Reconnect the retentate silicone (translucent) tubing to the
RET IN port. Turn pump on and set pump speed to 2.
Note: Check system connections for leaks—tighten connec-
tions if required
8. Set pump speed to 2. Recirculate for 15–30 minutes. Turn
pump off.
RETENTATE TUBING
Figure 26: Setup for system cleaning steps 7 & 8
Drain System
1. Disconnect pump outlet Sta-pure (white) tubing from pump
outlet port and place in waste collection vessel.
2. Disconnect retentate silicone (translucent) tubing from RET
IN port. Fluid should now drain by gravity. If additional
drainage is required, a syringe can be placed on the end of the
retentate tube and fluid can be blown down.
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