Mindray BS-180 - Page 253

10 Calculation Methods
10-10
calibration method are the same with that of Logit-Log 4P, but this method has a higher
fitting.
Exponential 5P
Calibration formula: ])(ln)(lnlnexp[
32
0
CcCbCaKRR +++=
This calibration method adopts five parameters:
0
R ,
K
,
a
, b and
c
.
This calibration method requires at least five calibrators. The concentration (or activity) of
calibrator 1 is 0, and the corresponding
R
is equal to
0
R
0.
This calibration method is
applied to the calibration curve that the response increases sharply when the
concentration reaches a specific value. SeeFigure 10-9.
Figure 10-9 Exponential 5p calibration curve
R
C
C1 C2 C3 C4 C5
Polynomial 5P
Calibration formula:
3
0
2
00
)
100
()
100
()
100
(ln
RR
d
RR
c
RR
baC
−
+
−
+
−
+=
This calibration method adopts five parameters:
0
R ,
a
, b ,
c
and d .
This calibration method requires at least five calibrators. The concentration (or activity) of
calibrator 1 is 0, and the corresponding
R
is equal to
0
R .
Parabola
Calibration formula: cbCaCR ++=
2
This calibration method adopts three parameters:
a
, b and
c
.
This calibration method requires at least three calibrators. The calibration parameters can
be calculated through the method of polynomial least squares.
Spline
Calibration formula:
32
0
)()()(
iiiiiii
CCcCCbCCaRR −+−+−+=
This calibration method requires 2 to 6 calibrators. The number of calibrators is set to be n,
so the calibration method has 4(n-1) parameters in total:
i
R
0
,
i
a ,
i
b and
i
c .