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Nikon ECLIPSE 80i - Page 21

Nikon ECLIPSE 80i
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Chapter 1 Part Names and Microscopy Procedures (Bright-Field Set)
1.3 Bright-Field Microscopy
19
1
5
Raise the condenser to the
uppermost position.
6
Fully open the field diaphragm
and aperture diaphragm.
(When using the 1-100x condenser, move the top
lens into the optical path.)
7
Set the 10x objective into the
optical path.
8
Set a specimen and move the
portion to be viewed into the
optical path.
Set a specimen and secure in
place using the specimen holder.
Move the portion to be viewed into the
optical path using the XY stage knobs.
Fully open the
aperture diaphragm
using the aperture
diaphragm lever.
Fully open the field
diaphragm using the
field diaphragm knob.
Raise the condenser using the
condenser focus knob.
Select
the 10x
objective.

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