64
Troubleshooting
6
If the microscope does not function property, take appropriate action as described below. If the
problem is still not resolved after referring to "Troubleshooting," please contact your nearest
Nikon representative
6.1
Optical
Problem Possible causes Remedy
Parts installed incorrectly
Install the Parts (nosepiece, condenser,
field diaphragm unit, aperture diaphragm
unit, etc.) correctly.
Movable parts not switched correctly
Switch the parts (optical path switching
lever, excitation method switching turret,
nosepiece, filter slider, condenser turret,
etc.) correctly. (Operate the parts to a
position until you feel resistance.)
Condenser adjusted in correctly
Adjust the condenser so that the field
diaphragm image is shown in the center
of the viewing field.
Improper objective-condenser
combination
Use a proper combination.
Lamp installed incorrectly
Install the lamp securely and adjust it as
necessary.
View field vignetting
Uneven illumination
across the view field
View field not visible
Field diaphragm not sufficiently
opened
Open the field diaphragm to the proper
setting.
Dirt and dust on lens, condenser,
eyepiece lens, filter and specimen
Clean.
Field diaphragm image not focused
on the specimen surface
Focus and center the condenser.
Dirt or dust in the field
of view
Field diaphragm closed too far
Open the field diaphragm to the proper
setting.
Field diaphragm closed or opened
too far
Open or close the field diaphragm to the
proper setting
Objective correction ring not
adjusted
Adjust the correction ring to match the
cover glass.
Condenser maladjusted
Adjust the condenser so that the field
diaphragm image is shown in the center
of the viewing field.
Cover glass unattached
Cover glass thickness not as specified
Attach a cover glass of the specified
thickness (0.17 mm).
Tip pf the oil-immersed objective
not immersed in oil
Unspecified immersion oil used
Use Nikon immersion oil.
Air bubbles in immersion oil Remove the air bubbles.
Immersion oil attached to tip of the
dry objective
Clean.
Poor image quality
Poor contrast
Poor resolution
Dirt or dust on lens, condenser, eye
piece lens, filter and specimen
Clean.
Revolving nosepiece not installed
correctly, or not rotated to the click
stop position
Install correctly and rotate to the click
stop position.
Uneven focus
Specimen projecting from the stage
surface
Securely attach the specimen to the
stage specimen holder.
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