Nikon ECLIPSE 80i - Epi-fluorescence Microscopy

Chapter 3 Part Names and Microscopy Procedures(With DIH-M Mounted)
3.3 Bright-Field Microscopy
42
5
Rotate the excitation method
switchover turret to set it a
position where no fluorescent
cube is installed.
6
Raise the condenser to the
uppermost position.
7
Fully open the field diaphragm
and aperture diaphragm.
8
Set the 10x objective into the
optical path.
Fully open the aperture
diaphragm using the
aperture diaphragm
lever.
Fully open the field
diaphragm using the
field diaphragm knob.
Raise the condenser using the
condenser focus knob.
Select the 10x ob
j
ective.
Rotate the excitation method
switchover turret.