Nikon ECLIPSE 80i - Page 46

Chapter 3 Part Names and Microscopy Procedures(With DIH-M Mounted)
3.3 Bright-Field Microscopy
44
12
Focus and center the condenser.
Refer to "11. Adjustment of Condenser Position" in
the separate Reference manual.
13
Switch to the desired objective
and view the specimen.
Adjust the field diaphragm and
aperture diaphragm each time
you change objectives.
F
ield dia
p
hra
g
m:
Set slightly narrower than the margins of
the field of view.
Ap
erture dia
p
hra
g
m:
70% to 80% of the maximum numerical
aperture (N.A.) of the objective
14
Turn off power after completing
observations.
Select the desired ob
j
ective.
Press the power switch
to the "◯" position.
Center the condenser
using the condenser
centering screws.
Focus the condenser using
the condenser focus knob.
Aperture diaphragm
lever
Field dia
p
hra
g
m knob
Field dia
p
hra
g
m
Eyepiece field of view
Field diaphragm
Eyepiece field of view