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Measure submenu
->Assign XY calibrations (images collected here are
generally pre-calibrated)
-> for OpticalDensity work
Thresholds define signal vs non-signal for
counts/measurements; auto method good starting point
Cut/join allows manual splitting/joining of objects for
counts/etc
Object standard used to define average object (to calc
objects that are touching w/o split)
IMA sophisticated measure/count tool (more later)
Use to define objects in objects
Active display of active ROI (or window) stats
Measure one/all ROI in one/all planes all at once
Displays intensity plot for ROI (can average)
Journal submenus
Keystroke recorder to automate mind-numbing tasks
Command strings are called “journals”
Menu to select and execute journal
(tools to help create complex protocols)
(Taskbars are main menus)
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