26
Refer to the MM section on filter definitions
currently used. Create a new name, select correct filter
cube position from list. This example is not correct
since the Sutter Lambda wheel positions are not
defined (and must be). Wheel B is the emission wheel,
Wheel A is the excitation wheel. If this was 380 setting
for Fura-2, Wheel A should be checked and set to
380/nd1 filter. Wheel B should be set to OPEN.
(confused? Ask for help!) Finally, set the Lambda
shutter A to ‘active’ (open only for actual image
collection). Now hit Add/replace. Once defined, the
values are stored until someone alters them. If we have problems with users altering main definitions, MF
can be locked into Administrator/multi-user mode to prevent this. PLEASE create versions vs replace!
Now you may want to setup the experiment details using Cfg Expt sidemenu or main Configure
menu. You can set the collection intervals per
wavelength, save intervals and screen update
intervals. To run fast, you might display (a slow
step) every 3 image. To run even faster (takes about
50msec to change filter positions) you can collect
one of the wavelengths (like 340 for Fura-2) every
other or every ten F380 images (software uses old
value until refreshed). Highest speeds require
streaming (no display at all), short exposures (will
limit rate) and locking the shutter open (see config
acquisition, config illumination)
Run the experiment using the Control menu
(open it using top sidemenu button if missing).
You probably must use the Focus routine to turn on
illumination, find cells by eye, MANUALLY SWITCH TO CAMERA
PORT, then hit Start Focusing.
Hit ‘start focusing’ to activate
camera (or toggle shutter to find cells by
eye). Note you can change binning and
exposures in this dialog (in case you
guessed wrong), test the stored values, or
store the current values into the ‘cfg acq’
settings with buttons here. Check other
wavelengths by clicking on the list
entries. Note subarray controls.
PDF Created with deskPDF PDF Writer - Trial :: http://www.docudesk.com
To Next Page
Loading...
