REPLIGEN XCell ATF 2 - Installing the Diaphragm; Figure 2. Xcell ATF 2 Stainless Steel Housing

XCell® ATF 2 and 4 Stainless Steel Housings
User Guide
10
SG-SSATF24 rev 1
6. XCell® ATF 2 Stainless Steel Housing
XCell® ATF 2 Stainless Steel Housing (Figure 2) is recommended for use with 2 - 10 L bioreactors. The
assembly of the housing and accessories includes installing the diaphragm into the housing,
connecting the hollow fiber filter to the housing, and connecting the filter to the bioreactor and the
harvest vessel. The system is prepared for use by filter wetting, integrity testing, and autoclaving.
F
igure 2. XCell® ATF 2 Stainless Steel Housing
6.1 Stainless steel XCell® ATF 2 Housing assembly
XCell® ATF 2 Stainless Steel Housing assembly includes installation of the diaphragm and hollow fiber
filter and connection of the tubing from the housing to the bioreactor and the housing to the harvest
vessel.
6.1.1 Installing the diaphragm
Important: The silicone diaphragm must be replaced after each run and prior to sterilization. To
avoid premature failure, it is important to ensure that the diaphragm is seated in the correct
orientation. Use with aqueous liquids only. For use with other fluids contact Repligen for a technical
discussion. When used as instructed, the diaphragm is designed to last over one million
pressure/exhaust cycles.
1. Hollow fiber membrane
2. Permeate/harvest
3. Retentate
4. Diaphragm pump
5. Air inlet
6. Drain port