REPLIGEN XCell ATF 2 - Removing the Housing from the Bioreactor; HFF Removal; Decontaminating the HFF for Disposal; Pump Air Inlet Filter, O-Rings, Gaskets, and Quick Connects

XCell® ATF 2 and 4 Stainless Steel Housings
User Guide
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SG-SSATF24 rev 1
4. Attach bottom permeate (C2) to hose barb.
5. Attach drain (C3) to hose barb. Autoclave.
6.5 Disassembly and maintenance
6.5.1 Removing the housing from the bioreactor
1. Stop the permeate/harvest pump and disconnect the harvest bag from the housing.
2. Stop and disconnect the A2C line from the diaphragm pump.
3. Close or disconnect compressed air and vacuum services from the pneumatic enclosure.
4. Disconnect sensors from the filtration assembly to pneumatic enclosure.
5. If necessary, remove the pneumatic enclosure from the filtration assembly.
6. Close valves connecting the filtration assembly to the vessel.
7. Drain liquid from the connection.
8. Optionally, purge the connection with water, steam, or some other medium.
9. Disconnect the filtration assembly from vessel.
10. Remove the filtration assembly to an appropriate work area.
6.5.2 HFF Removal
1. Drain the system into an appropriate waste vessel or drain.
2. Remove all sensors from the housing.
3. Remove air inlet filter connected to the diaphragm pump and any other connections to the
XCell® ATF Housing.
4. Open the clamp connecting the filter to the diaphragm pump and separate.
5. Open the clamp connecting the filter to the A2B tubing and separate.
6. Remove the O-ring at the exposed end of the HFF.
7. Press the HFF in the opposite direction to remove.
8. Prepare filter housing for cleaning and reuse.
6.5.3 Decontaminating the HFF for Disposal
1. Rinse the HFF two times with water to remove residual cells and debris.
2. Decontaminate with either 0.5M NaOH, potassium hydroxide, or bleach for 2 – 24 hours.
Note: 5.25% chlorine is equivalent to 52,500 parts per million (ppm). If household chlorine bleach is
used, two working solutions are generally recommended: 5000 - 6000 ppm for initial
decontamination of organic spill material, and 500 - 600 ppm for disinfection of cleaned
surfaces.
6.5.4 Pump Air Inlet Filter, O-Rings, Gaskets, and Quick Connects
All consumable items are single use.