REPLIGEN XCell ATF 2 - Xcell ATF 4 Stainless Steel Housing Hollow Fiber Filter Installation; Figure 8. Orientation of Silicone Diaphragm in Xcell ATF 4 Stainless Steel Housing

XCell® ATF 2 and 4 Stainless Steel Housings
User Guide
17
SG-SSATF24 rev 1
Figure 8. Orientation of silicone diaphragm in XCell® ATF 4 Stainless Steel Housing
7.1.1.1 XCell® ATF 4 Stainless Steel Housing diaphragm placement
1. Set the pump air-side (PA) hemisphere (Figure 8
, 2) on a flat surface with the open (concave)
side of the hemisphere facing up.
2. Place diaphragm (3) with the convex side oriented up onto the PA hemisphere, and place
the PL hemisphere, wide opening down, on top of the diaphragm.
3. Assure the diaphragm gasket O-ring is positioned uniformly around the periphery of the
pump O-ring groove. The two hemispheres, with diaphragm in between, should be equally
spaced.
4. Clamp (4) the two hemispheres together.
5. Secure the diaphragm pump to the stand.
7.1.2 XCell® ATF 4 Stainless Steel Housing hollow fiber filter installation
XCell® ATF 4 Stainless Steel Housing accommodates a variety of hollow fiber filters. For support in
selecting hollow fiber filters, please contact your local Repligen Field Applications Specialist (FAS).
1. Stand the filter housing vertically, with harvest port up.
2. Insert the HFF into the housing.
3. Insert one O-ring into the groove between HFF and housing end-ferrule wall.
4. Place the reducer onto the end, forcing the O-ring into the groove.
5. Clamp the reducer to the housing, compressing the O-ring against the HFF. A seal is formed
between the housing, reducer and HFF.
6. On the other end of the filter housing, place the second O-ring into the groove between HFF
and housing end-ferrule.
7. Carefully place the filter housing onto the diaphragm pump, forcing the O-ring deeper into
the groove.
8. Clamp the filter housing to the diaphragm pump.
9. Assemble the rest of the XCell® ATF Housing, including tubing set kits.
7.2 Filter preparation
To prepare the filter for use, the assembled housing should be pressure tested, wetted, integrity
tested and autoclaved.
1. Housing assembly, pump liquid-side
(PL) hemisphere
2. Housing assembly, pump air- side
(PA) hemisphere
3. Diaphragm
4. Clamp