XCell® ATF 2 and 4 Stainless Steel Housings
SG-SSATF24 rev 1
1. Place a sanitary gasket (Figure 4, 3) into the sanitary groove on top of the Pump Liquid-side
(PL) hemisphere (4).
2. Connect the HFM (1) to the pump housing by aligning it with the sanitary gasket on the top
of the PL hemisphere (4).
3. Secure the connection tightly with a ¾" tri-clamp (2).
6.3 Filter preparation
To prepare the filter for use, the assembled housing should be wetted, integrity tested and
autoclaved.
6.3.1 Filter wetting
Hollow fiber filters require wetting after installation and pressure testing. Slight variations in
assembly are possible depending on the configuration of equipment purchased and its intended use.
Refer to the XCell® ATF Technology Filter Preparation and Autoclaving User Guide for instructions.
6.3.2 Integrity testing pre-autoclaving
Note: Repligen strongly recommends the practice of pre-autoclaving integrity testing to ensure a
proper assembly prior to autoclaving.
Refer to the XCell® ATF Technology Filter Preparation and Autoclaving User Guide for configurations
for both glass and single-use bioreactor integrity tests and testing instructions and criteria.
6.3.3 Autoclaving
The XCell® ATF 2 Stainless Steel Housing assembly is autoclaved either before or after integrity
testing. XCell® ATF 2 comprises a small-diameter, self-contained hollow fiber filter connected to a
diaphragm pump, and can be autoclaved using a pre-programmed liquid cycle at 121°C for 30
minutes. Refer to the XCell® ATF Technology Filter Preparation and Autoclaving User Guide for
instructions.
6.3.4 Integrity testing post-autoclaving
Post-autoclaving integrity testing is required only if testing was not performed pre-autoclaving. Refer
to the XCell® ATF Technology Filter Preparation and Autoclave User Guide for instructions.
6.4 Connecting XCell® ATF 2 Stainless Steel Housing to a bioreactor
XCell® ATF 2 Stainless Steel Housing is generally connected to the bioreactor prior to autoclaving.
Fluid management for XCell® ATF 2 Stainless Steel Housings include retentate (A2B) tubing set kits
and accessories that connect the housing with the bioreactor, ensuring proper exchange of cell
culture material. The A2B tubing connects to XCell® ATF 2 with a tri-clamp. The bioreactor end of the
tubing is available with either a tri-clamp or an AseptiQuik® or Readymate™ disposable aseptic
connector. The tubing is connected to the headplate (Table 5
) and feeds into a dip tube (P/N TC-DIP-
LAB-.25/.5-250 or TC-DIP-.25/.5-250. Permeate tubing connects to the housing with a hose barb and
should be sterilized by autoclave or attached to the harvest vessel with a tubing welder or
disposable sterile coupling. The permeate tubing should be compatible with the permeate/harvest
pump. Tubing is also provided for the bottom permeate and drain ports, both of which connect with
a hose barb.
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