XCell® ATF 2 and 4 Stainless Steel Housings
SG-SSATF24 rev 1
1. Introduction
XCell® ATF Technology uses alternating tangential flow (ATF) to intensify upstream processes by
retaining cells in suspension cultures, such as mammalian cell culture and viral vectors. An
innovative diaphragm pump creates alternating tangential flow, leading to high viable cell densities
and increased throughput with lower cost of goods. Typical applications include intensification of
the following processes:
• N-1 fed-batch
• Long-term continuous processing
• Vaccine and virus production
• Gene therapy and media exchange
XCell® ATF 2 and XCell® ATF 4 Stainless steel Housings enable production of high density, high
viability cell culture applications with linear flux and low shear for scale-up to 50 L. The stainless
steel product line includes the housings, XCell® Lab Controllers, software, tubing set kits, pressure
sensors, and accessories.
Hollow fiber filters (HFF) used in XCell® ATF Technology are made of Polyethersulfone (PES) or
polysulfone (PS) and are available in 0.2 and 0.5 µm and 50 kD pore size and 1 mm inner diameter
and are scalable across device sizes.
Single-use tubing set kits and accessories are available for the connection of XCell® ATF 2 and 4
Stainless Steel Housings to both glass and single-use bioreactors, simplifying preparation and
connectivity and allowing for the proper exchange of cell culture material. XCell® Lab Controllers
operate the diaphragm pumps. The housings and tubing set kits are also compatible with legacy C24
Controllers. Additional information on the use of the controllers and XCell® ATF Technology can be
found in XCell® Lab Controller User Guide.
This document describes the major components, set-up, connectivity, and preparation of the glass
XCell® ATF 2 and 4 Housings and hollow fiber filters. Users should possess a basic level of skill in the
areas of aseptic technique and fluid handling.
For further support in optimizing or troubleshooting, please contact your local Repligen Field
Applications Specialist (FAS).
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