Sartorius Tacta - 6. Operating the Pipette; 6.1 Good Pipetting Practices; 6.2 Forward Pipetting

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6. Operating the Pipette
The Tacta® pipette is generally used for forward and reverse pipetting.
6.1 Good Pipetting Practices
− Make sure the tip is firmly attached to the tip cone(s).
− Pre-rinse the tip before aspirating by filling and emptying the tip three to five times. This is particularly important when pipetting
liquids with a viscosity and density greater than water, or volatile liquids with high vapor pressure (such as ethanol).
− Make sure the pipette, tip, and liquid are at the same temperature.
− Hold the pipette upright when aspirating, and only place the tip a few millimeters into the liquid (2-3 mm if the pipette’s volume
is 3-2000 μl; 5-6 mm if the volume is 5-10 ml).
− Always press and release the operating button slowly and smoothly.
− When pipetting a liquid that is not at the ambient temperature, change the tip after each pipetting. Do not rinse the tip before
use.
− To avoid contaminating the pipette’s housing with either the sample in the tip or the work surface, do not lay the pipette on its
side.
− Never strike the tip cone against the tip tray.
− When handling infectious or radioactive agents, wear appropriate protective gear and take all sensible precautions.
− Avoid exposing the unit to extreme temperature changes, humidity, or dust. Operating temperatures should be between 15 and
40°C.
6.2 Forward Pipetting
Forward pipetting is the most common pipetting technique. It uses a blow-out function to ensure complete delivery of the liquid.
This technique aspirates and then dispenses the selected liquid volume. It is recommended for aqueous liquids, liquids containing small
amounts of detergents or proteins, and solvents.
1. Fit the tip onto the pipette tip cone.
2. Press the operating button to the first stop.
3. Place the tip just under the surface of the liquid and smoothly release the operating button, allowing it to return to to the starting
position. Wait one second.
4. Carefully withdraw the tip from the liquid, touching it against the edge of the container to remove any excess liquid.
5. Dispense the liquid by pressing the operating button to the first stop.
6. After a short delay, press the operating button to the second stop. This empties the tip.