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8.2 Adjusting the Pipette
Your Tacta® pipette has been factory-checked using the forward pipetting technique and certified at 22°C using grade-3 distilled
water according to ISO 3696. The calibration is based on ISO 8655-6: Gravimetric test method for volumetric instruments.
In the following situations factory calibration is not applicable and the pipette will need adjusting:
− When pipetting liquids which differ from water in density, viscosity, vapor pressure, or other quality.
− When reverse pipetting
− When the geometry of the tip used clearly differs from standard tip geometry.
− When the pipette is used at high altitudes.
To adjust the pipette:
1. Use the tool to open the cover above the adjustment window.
2. Place the star head of the adjustment tool into the socket, and press firmly. This releases the lock.
3. Turn the adjustment tool counterclockwise to decrease the volume and clockwise to increase it.
NOTE: When adjusting multichannel models, hold the pipette by the handle. The lower part of the pipette (the tip cone housing) will
move during adjustment – allow it to move freely to prevent breaking the pipette.
Table 2: Volume adjustment units in μl
Tacta® model Channels Increment (μl) -45 -10 -5 -1 0 1 5 10 45
0.1-3 μl 1 0,002 -0.09 -0.02 -0.01 -0.002 0 0.002 0.01 0.02 0.09
0.5-10 μl 1 0,01 -0.45 -0.1 -0.05 -0.01 0 0.01 0.05 0.1 0.45
2-20 μl 1 0,02 -0.9 -0.2 -0.1 -0.02 0 0.02 0.1 0.2 0.9
10-100 μl 1 0,1 -4.5 -1 -0.5 -0.1 0 0.1 0.5 1 4.5
20-200 μl 1 0,2 -9 -2 -1 -0.2 0 0.2 1 2 9
100-1000 μl 1 1 -45 -10 -5 -1 0 1 5 10 45
500-5000 μl 1 5 -225 -50 -25 -5 0 5 25 50 225
1-10 ml 1 10 -450 -100 -50 -10 0 10 50 100 450
0.5-10 μl 8 and 12 0,01 -0.45 -0.1 -0.05 -0.01 0 0.01 0.05 0.1 0.45
5-100 μl 8 and 12 0,1 -4.5 -1 -0.5 -0.1 0 0.1 0.5 1 4.5
30-300 μl 8 and 12 0,2 -9 -2 -1 -0.2 0 0.2 1 2 9
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