2
C
Flow
™
Space
Operatin
Manual
March 2021
Table of contents
1 Identification ............................................................................................................ 5
1.1 Product information ......................................................................................... 5
1.2 Manufacturer ..................................................................................................... 5
2 Introduction ............................................................................................................. 6
2.1 What is the CyFlow™ Space? ......................................................................... 6
2.2 What are the applications for which the CyFlow™ Space can be used? .... 6
2.3 What topics are covered by this manual? ...................................................... 6
2.4 What other manuals are available? ................................................................. 6
2.5 What should I know before operating the CyFlow™ Space? ....................... 6
2.6 What standards were applied for CE compliance? ....................................... 7
2.7 What typographical conventions are used in this manual? ......................... 7
2.7.1 Symbols in this document ................................................................................... 7
2.7.2 Safety information ............................................................................................... 7
3 Typical Steps of Particle Analysis ......................................................................... 9
3.1 Preparation and Staining ................................................................................. 9
3.2 Flow Cytometry Analysis ................................................................................. 9
3.3 Real-time Data Processing and Results ......................................................... 9
3.4 Absolute Cell Counting .................................................................................. 10
4 Safety ..................................................................................................................... 11
4.1 Laser radiation hazards ................................................................................. 11
4.2 Electrical hazards ........................................................................................... 11
4.3 Alterations to the device ................................................................................ 11
5 Operating Basics ................................................................................................... 12
5.1 Switching on the CyFlow™
Space ................................................................ 12
5.1.1 Check SHEATH and WASTE bottle ................................................................. 12
5.1.2 Switch on the instrument .................................................................................. 12
5.1.3 Control of the 488 nm laser output power (for 200 mW high power laser only,
model Coherent Sapphire) ........................................................................................... 12
5.1.4 Switch on peripheral devices ............................................................................ 12
5.1.5 Switch on computer .......................................................................................... 12
5.1.6 Start instrument operating software .................................................................. 13
5.1.7 Priming the instrument (initial cleaning) ............................................................ 13
5.2 Multi laser Measurements .............................................................................. 13
5.3 Starting a Measurement ................................................................................. 14
5.4 Switching off the CyFlow™ Space ................................................................ 16
6 True Volumetric Absolute Counting – Overview ................................................ 17
6.1 Non-volumetric Flow Cytometric Techniques for Absolute Counting ...... 17
6.2 True Volumetric Absolute Counting ............................................................. 17
6.3 Precision and Reproducibility ....................................................................... 18
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