22
C
Flow
™
Space
Operatin
Manual
March 2021
FL5: red fluorescence II (APC)
FL6: blue fluorescence (CFP, Alexa 405)
Figure 5: Exemplary demonstration of the optical bench of a CyFlow™ Space with 2 lasers and 8
parameters (left) and 3 lasers and 8 parameters (right). Other laser light sources and parameter
allocations are possible. D = detector (photo multiplier)
7.3 Other parameter allocations possible
The instrument configured as flow cytometer uses a second optical bench allocating 8
additional detectors extending the number of detectors to 16.
Information on the optical configuration of a specific instrument is displayed in the
optical layout scheme of an instrument, which can be found in the individual
instruments documentation.
The instrument is equipped with a CMOS camera in order to monitor the particle flow and
to check the focus of the objective (systems with 16 detectors come without CMOS
camera!).