12
C
Flow
™
Space
Operatin
Manual
March 2021
5 Operating Basics
5.1 Switching on the CyFlow™
Space
5.1.1 Check SHEATH and WASTE bottle
Make sure SHEATH bottle is filled with no more than 1600 ml of Sheath
Fluid and is closed tightly with the screw top. Tilt sheath bottle in order
to release air bubbles trapped in the yellow inline filter!
Make sure the WASTE bottle is empty.
It is recommended to replace the sheath fluid at least once a week or before any daily use.
5.1.2 Switch on the instrument
The CyFlow™ Space Flow Cytometer is operated with 100- 240 V AC.
Switch on main power at the left side of the instrument. The 488 nm
laser has its own power button close by. Also switch on the 488 nm
laser, please see chapter 10.2 Instrument Setup – Step by Step, Figure:
13.
638/640 nm lasers are software controlled or are switched by additional
labelled hardware switches next to the 488 nm laser power switch, e.g.
the 405 nm, the 375 nm and the 532 nm lasers.
5.1.3 Control of the 488 nm laser output power (for 200 mW high power laser only,
model Coherent Sapphire)
The 488 nm 200 mW high power laser can be adjusted in its operation
power between 50 and 200 mW. For power selection please double click
the desktop icon shown on the left (488-200).
To change selected laser power please type in:
p=value enter
To inquire about actual selected laser power type in:
?p enter
5.1.4 Switch on peripheral devices
Switch on printer and/or sorter module.
5.1.5 Switch on computer
Finally, switch on computer by the Computer Power switch.
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