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C
Flow
™
Space
Operatin
Manual
March 2021
Example: Assume vertebrate leukocytes in full blood are to be analysed. Triggering on a
scatter parameter would be difficult due to the high number of erythrocytes in the same
sample. Staining with a DNA dye (e.g. PI) and triggering on the DNA parameter will
discriminate the leukocytes containing a nucleus from erythrocytes without a nucleus. This
works even though there are orders of magnitudes more erythrocytes than leukocytes in
the sample.
Trigger All
The CyFlow™ Space offers an additional method for triggering which allows to trigger on
all parameters.
Example: Assume a part of the particles of interest are exclusively red and another part
exclusively green fluorescing. Triggering on the green fluorescence would exclude the red
particles from the analysis, triggering on red would exclude the green. If the interest is in
both subpopulations, it is required to trigger on green and red simultaneously. This can be
done with Trigger All.
Trigger parameter(s) and mode can be selected in the instrument settings box. Please see
“FloMax
®
– Acquisition and Instrument Control” for details.
9.1.2 Gain
NOTICE
Gain and lin/log amplification mode cannot be adjusted after the
acquisition.
Be sure not to use too high gain values. Increasing the gain values too
much can cause a decrease of amplification or a loss of signals due to
saturation effects.
The gain value does not necessarily be equal to the PMT high voltage
nor does it be proportional to the amplification.
Adjustment of gain
By means of the gain, the signal amplification can be individually adjusted in a wide range
for each parameter. For the used photomultiplier tubes (PMTs) by changing the gain value,
the PMT's high voltage is adjusted.
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