CyFlow
™
Space | Operating Manual | March 2021
43
Optical Coupling Standard objective mount with high
numerical aperture
Excitation Optics Elliptical 15 µm x 60 µm at 488 nm
NOTICE
Information on the optical configuration of a specific instrument is
displayed in the optical layout scheme of an instrument which can be
found in the individual instruments documentation.
12.3 Fluidics
Flow Cuvette Synthetic quartz flow cuvette (350x 200
µm) for laminar sample transport with
sheath fluid for fluorescence, forward and
side scatter light detection
Sample Delivery
Computer controlled precision syringe
pump for contamination-free sample
transport.
Built-in air pressure for sheath fluid. Sheath
fluid pressure is adjustable from 0-300
mbar (Computer controlled). Default
setting: 200 mBar.
Sampling Volume Continuous up to 1000 µl
200 µl for precision absolute counting
Free sampling volume with syringe
counting method
Flow Rates 1) Sample volume speed adjustable
continuously between 0 and 20 µl/s
2) Sheath fluid pressure continuously
adjustable
Fluidics Volume 2 x 2-litre reservoirs for sheath fluid and
waste
Biosafety System Avoids sample droplets and sample cross
contamination
12.4 Electronics and Computer
Electronics Parallel real-time signal processing for
each of the optical channels with selectable
linear, 3- or 4-decade logarithmic
amplification (6-decade log amplifiers),
pulse height, area and width analysis for
doublet discrimination, 16 bit analogue-to-
digital converters, trigger on any parameter
or all parameters
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