SYSMEX CyFlow Space - Optical Configuration and Parameters; Two-Laser Instrument Setup; Three-Laser Instrument Setup

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CyFlow

™

Space | Operating Manual | March 2021

7 Optical Standard Setup – Parameters

NOTICE

If required by a specific application, the optical standard setup can be

optimized by exchanging preassembled removable mirror/filter blocks.

This is a matter of seconds and does not require readjustments.

The CyFlow™ Space flow cytometer can be configured as flow cytometer or as a flow

cytometer with cell sorting option. The configuration as flow cytometer can accommodate

1 to 4 laser light sources, a UV-LED, different laser power intensities and up to sixteen

optical parameters.

The configuration as flow cytometer with cell sorting option can accommodate 1 to 3 laser

light sources, a UV-LED, and up to eight optical parameters.

Due to its modular concept the optical configuration can be adopted to many different

purposes. Exemplary standard configurations e.g. include:

7.1 2 Laser instrument

Instrument is equipped with a blue diode pumped solid-state Laser (50 mW) at 488 nm and

a red laser diode at 638 nm (25 mW). Up to eight optical parameters can be included

(colours reflect laser origin of the signal).

FSC: forward scatter

SSC: side scatter

FL1: green fluorescence (FITC)

FL2: orange fluorescence (PE)

FL3: red fluorescence I (PE-Cy5)

FL4: far red fluorescence I (PE-Cy7)

FL5: red fluorescence II (APC)

FL6: far red fluorescence II (APC-Cy7)

7.2 3 Laser instrument (high power laser 488 nm)

Instrument equipped with a blue diode pumped solid-state Laser (200 mW) at 488 nm, a

violet laser diode at 405 nm (100 mW) and a red laser diode at 638 nm (25 mW). In a

configuration as flow cytometer with cell sorting option this instrument accommodates up