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Tissue-Tek TEC 5100 - Sample Embedding Workflow Steps

Tissue-Tek TEC 5100
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10. Move the base mold to the hot plate under
paraffin dispenser.
11. While holding the cassette under paraffin dis-
penser (and above center hot plate), press fin-
gerplate or use optional foot pedal to dispense
paraffin into base mold until the cassette is
filled with paraffin.
NOTE: Do not overfill base mold.
12. Place base mold containing embedded tissue(s)
onto cooling plate.
NOTE: The surface of the cooling plate will
usually be covered with a light layer of frost
(depending on ambient humidity). This
enhances heat exchange between the warmed
base mold and the cooling plate.
13. Check to make sure the paraffin block is com-
pletely solidified; then remove paraffin block
from base mold.
NOTE: The paraffin block should easily release
from the base mold. If not, the base mold may
need cleaning. Use of the Tissue-Tek
®
Mold
Release Concentrate (product code 4141) will
make demolding easier.
14. If the paraffin block is not to be sectioned
immediately, the block may be stored on the
cooling plate.
15. Press LIGHT key to turn off Work Light.
NOTE: To extend life of light bulbs, always
turn off Work Light after embedding procedure
is completed.
5.2 Revised 07/12/11
EMBEDDING PROCEDURE

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