CHAPTER 3 BASIC OPERATION OF THE MINI-PAM
3 Basic Operation of the MINI-PAM
The MINI-PAM is very easy to operate. It has a two-line LC-
display and a small tactile keyboard with eight function keys (ON,
OFF, MODE, MEM, ∧, ∨, START, SET). In order to get started, only
the fiberoptics have to be connected and the ON-key is pressed. Now
the system is ready for recording fluorescence yield of any sample
which is close (5-20 mm) to the free end of the fiberoptics. The
actual measurement of the most relevant YIELD-parameter (quantum
yield of photochemical energy conversion) just involves pressing the
START-key. Then on the display, for example, the following
information is shown:
1: 445F 1739M ..C
F: 448 745Y ..E ..L
The meaning of the various displayed parameters is as follows:
1: Number denoting the standard MODE-menu position 1
which is automatically installed whenever the MINI-PAM
is switched on or a YIELD-determination is carried out
via START.
445F Fluorescence yield (F) measured briefly before the last
saturating light pulse triggered by START.
1739M Maximal fluorescence yield (M = Fm or Fm') measured
during the last saturating light pulse triggered by START.
..C Temperature in degree Celsius, display of which requires
optional Leaf-Clip Holder 2030-B.
F: 448 Momentary fluorescence yield displaying small
fluctuations.
4
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